ROMIEU Anthony Voir la fiche profil en français

Engineer in chemistry from the National Higher Chemistry School of Montpellier (graduated in June 1995). From October 1996 to April 1999, i did a Ph.D. iunder the guidance of Drs. J. Cadet and D. Gasparutto (laboratory of Nucleic Acids Damages, CEA-Grenoble) where i worked on the synthesis and biophysical characterizations of modified oligonucleotides bearing altered nucleosides (related to oxidative stress). After serving for two years as CNRS study engineer in the lab of Prof. B.-P. Roques at the University Paris 5, in the fields of medicinal chemistry and peptide synthesis, i joined in June 2001 the private biotech company Manteia Predictive Medicine (located in Switzerland and spin-off of the Serono group) as a senior scientist in bioorganic chemistry. The core business of this company was devoted to the development and commercialization of an original high-throughput DNA sequencing technology. Following a restructuring, leading to the acquisition of Manteia by Solexa Ltd. (this latter company will become a wholly owned subsidiary of Illumina, Inc. in January 2007), i was appointed as lecturer in bioorganic chemistry at the University of Rouen. From February 2004 to August 2013, i co-facilitate with Prof. P.-Y. Renard the bioorganic chemistry research team from the COBRA lab (UMR CNRS 6014). In September 2013, i was appointed as a full professor at the University of Burgundy and a junior member of the French University Institute (IUF). I also joined the ICMUB laboratory (UMR CNRS 6302) and my current research interests mainly focus on the development of advanced chemical tools ("smart" optical (bio)probes, novel fluorogenic reactions, and cross-linking reagents for multiple bioconjugation) for biosensing and bioimaging applications. I'm co-author of more than 90 scientific papers and co-inventor of over a dozen patents; three of them are actively being pursued by the Illumina company ("Genome Analyzer" DNA sequencing technology).

(1) Development of high-performance engineered organic-based fluorophores for biological imaging.
(2) "Covalent-assembly" type probes for biosensing/bioimaging.
(3) Novel multivalent molecular platforms for rapid multiple bioconjugation.

[1] Ponsot F, Desbois N, Bucher L, Berthelot M, Mondal P, Gros CP, Romieu A. Near-infrared emissive bacteriochlorin-diketopyrrolopyrrole triads: Synthesis and photophysical properties Dyes Pigm. 2018: in press, see DOI: 10.1016/j.dyepig.2018.1008.1059.
[2] Dejouy G, Laly M, Valverde IE, Romieu A. Synthesis, stability and spectral behavior of fluorogenic sulfone-pyronin and sulfone-rosamine dyes Dyes Pigm. 2018; 159: 262-274.
[3] Debieu S, Romieu A. Kinetics improvement of protease-mediated formation of pyronin dyes Tetrahedron Lett. 2018; 59: 1940-1944.
[4] Berthelot M, Hoffmann G, Bousfiha A, Echaubard J, Roger J, Cattey H, Romieu A, Lucas D, Fleurat-Lessard P, Devillers CH. Oxidative C-N Fusion of Pyridinyl-Substituted Porphyrins Chem. Commun. 2018; 54: 5414-5417.
[5] Azarias C, Ponce-Vargas M, Navizet I, Fleurat-Lessard P, Romieu A, Le Guennic B, Richard J-A, Jacquemin D. Rationalisation of the Optical Signatures of nor-Dihydroxanthene-Hemicyanine Fused Near-Infrared Fluorophores By First Principle Tools Phys. Chem. Chem. Phys. 2018; 20: 12120-12128.
[6] Ong MJH, Debieu S, Moreau M, Romieu A, Richard J-A. Synthesis of N,N-Dialkylamino-nor-Dihydroxanthene-Hemicyanine Fused Near-Infrared Fluorophores and Their First Water-Soluble and/or Bioconjugatable Analogues Chem. - Asian. J. 2017; 12: 936-946.
[7] Debieu S, Romieu A. In situ formation of pyronin dyes for fluorescence protease sensing Org. Biomol. Chem. 2017; 15: 2575-2584.
[8] Chevalier A, Renard P-Y, Romieu A. Azo-based Fluorogenic Probes for Biosensing and Bioimaging: Recent Advances and Upcoming Challenges Chem. - Asian. J. 2017; 12: 2008-2028.
[9] Romieu A, Richard J-A. An expedient synthesis of N,N-dialkylamino-dihydroxanthene-pyrylium conjugated near-infrared fluorescent dyes Tetrahedron Lett. 2016; 57: 317-320.
[10] Ong MJH, Srinivasan R, Romieu A, Richard J-A. Divergent synthesis of dihydroxanthene-hemicyanine fused near-infrared fluorophores through the late-stage amination of a bifunctional precursor Org. Lett. 2016; 18: 5122-5125.
[11] Glowacka PC, Maindron N, Stephenson GR, Romieu A, Renard P-Y, da Silva Emery F. Synthesis and photophysical properties of iron-carbonyl complex-coumarin conjugates as potential bimodal IR-fluorescent probes Tetrahedron Lett. 2016; 57: 4991-4996.
[12] Abdulaeva IA, Birin KP, Michalak J, Romieu A, Stern C, Bessmertnykh-Lemeune A, Guilard R, Gorbunova YG, Tsivadze AY. On the synthesis of functionalized porphyrins and porphyrin conjugates via β−aminoporphyrins New J. Chem. 2016; 40: 5758-5774.
[13] Roubinet B, Massif C, Moreau M, Boschetti F, Ulrich G, Ziessel R, Renard P-Y, Romieu A. New 3-(Heteroaryl)-2-iminocoumarin-based Borate Complexes: Synthesis, Photophysical Properties, and Rational Functionalization for Biosensing/Biolabeling Applications Chem. - Eur. J. 2015; 21: 14589-14601.
[14] Roubinet B, Chevalier A, Renard P-Y, Romieu A. A Synthetic Route to 3-(Heteroaryl)-7-Hydroxycoumarins Designed for Biosensing Applications Eur. J. Org. Chem. 2015: 166-182.
[15] Roubinet B, Bailly L, Petit E, Renard P-Y, Romieu A. A FRET-based probe for fluorescence sensing of sulfide/sulfite analytes, using a novel long-wavelength water-soluble 7-hydroxycoumarin as reporter fluorophore Tetrahedron Lett. 2015; 56: 1015-1019.
[16] Romieu A. "AND" luminescent "reactive" molecular logic gates: a gateway to multi-analyte bioimaging and biosensing Org. Biomol. Chem. 2015; 13: 1294-1306.
[17] Nury C, Redeker V, Dautrey S, Romieu A, van der Rest G, Renard P-Y, Melki R, Chamot-Rooke J. A Novel Bio-Orthogonal Cross-Linker for Improved Protein/Protein Interaction Analysis Anal. Chem. 2015; 87: 1853-1860.
[18] Jacquemet A, Rihn S, Ulrich G, Renard P-Y, Romieu A, Ziessel R. Rational Design of Latent Fluorophores based on Water-­Soluble ortho-­Hydroxyphenyl-­triazine ESIPT Dyes and suitable for Lipase Sensing Eur. J. Org. Chem. 2015: 1664-1669.
[19] Heyer E, Lory P, Leprince J, Moreau M, Romieu A, Guardigli M, Roda A, Ziessel R. Highly fluorescent, water-soluble, bioconjugable diketopyrrolopyrrole dyes Angew. Chem. Int. Ed. 2015; 54: 2995-2999.
[20] Debieu S, Romieu A. Dual enzyme-responsive "turn-on" fluorescence sensing systems based on in situ formation of 7-hydroxy-2-iminocoumarin scaffolds Org. Biomol. Chem. 2015; 13: 10348-10361.
[21] Chevalier A, Renault K, Boschetti F, Renard P-Y, Romieu A. Rapid Synthesis of Unsymmetrical Sulforhodamines through Nucleophilic Amination of a Mono-Bromo Sulfoxanthene Dye Eur. J. Org. Chem. 2015: 152-165.
[22] Chevalier A, Piao W, Hanaoka K, Nagano T, Renard P-Y, Romieu A. Azobenzene-caged sulforhodamine dyes: a novel class of "turn-on" reactive probes for hypoxic tumor cell imaging Methods Appl. Fluoresc. 2015; 3: 044004.
[23] Roubinet B, Renard P-Y, Romieu A. New insights into the water-solubilization of thiol-sensitive fluorogenic probes based on long-wavelength 7-hydroxycoumarin scaffolds Dyes Pigm. 2014; 110: 270-284.
[24] Oueis E, Santoni G, Ronco C, Syzgantseva O, Tognetti V, Joubert L, Romieu A, Weik M, Jean L, Sabot C, Nachon F, Renard P-Y. Reaction site-driven regioselective synthesis of AChE inhibitors Org. Biomol. Chem. 2014; 12: 156-161.
[25] Jouanno L-J, Chevalier A, Sekkat N, Perzo N, Castel H, Romieu A, Lange N, Sabot C, Renard P-Y. Kondrat'eva Ligation: Diels‒Alder-based Irreversible Reaction for Bioconjugation J. Org. Chem. 2014; 79: 10353-10366.
[26] Jolivel V, Arthaud S, Botia B, Portal C, Delest B, Clavé G, Leprince J, Romieu A, Renard P-Y, Touzani O, Ligeret H, Noack P, Massonneau M, Fournier A, Vaudry H, Vaudry D. Biochemical Characterization of a Caspase-3 Far-red Fluorescent Probe for Non-invasive Optical Imaging of Neuronal Apoptosis J. Mol. Neurosci. 2014; 54: 451-462.
[27] Chevalier A, Renard P-Y, Romieu A. Straightforward synthesis of bioconjugatable azo dyes. Part 2: Black Hole Quencher-2 (BHQ-2) and BlackBerry Quencher-650 (BBQ-650) scaffolds Tetrahedron Lett. 2014; 55: 6764-6768.
[28] Chevalier A, Renard P-Y, Romieu A. Straightforward synthesis of bioconjugatable azo dyes. Part 1: Black Hole Quencher-1 (BHQ-1) scaffold Tetrahedron Lett. 2014; 55: 6759-6763.
[29] Chevalier A, Renard P-Y, Romieu A. Azo-Sulforhodamine Dyes: A Novel Class of Broad Spectrum dark Quenchers Org. Lett. 2014; 16: 3946-3949.
[30] Chevalier A, Renard P-Y, Romieu A. Straightforward Access to Water-Soluble Unsymmetrical Sulfoxanthene Dyes. Application to the Preparation of Near-Infrared Dyes with Large Stokes' Shifts Chem.--Eur. J. 2014; 20: 8330-8337.
[31] Besret S, Vicogne J, Dhamani F, Fafeur V, Desmet R, Drobecq H, Romieu A, Melnyk P, Melnyk O. Thiocarbamate-linked polysulfonate-peptide conjugates as selective hepatocyte growth factor receptor binders Bioconjugate Chem. 2014; 25: 1000-1010.
[32] Viault G, Dautrey S, Maindron N, Hardouin J, Renard P-Y, Romieu A. The first "ready-to-use" benzene-based heterotrifunctional cross-linker for multiple bioconjugation Org. Biomol. Chem. 2013; 11: 2693-2705.
[33] Romieu A, Massif C, Rihn S, Ulrich G, Ziessel R, Renard P-Y. The first comparative study of the ability of different hydrophilic groups to water-solubilize fluorescent BODIPY dyes New J. Chem. 2013; 37: 1016-1027.
[34] Priem T, Bouteiller C, Camporese D, Brune X, Hardouin J, Romieu A, Renard P-Y. A novel sulfonated prosthetic group for [18F]-radiolabeling and imparting water solubility of biomolecules and cyanine fluorophores Org. Biomol. Chem. 2013; 11: 469-479.
[35] Chevalier A, Mercier C, Saurel L, Orenga S, Renard P-Y, Romieu A. The first latent green fluorophores for the detection of azoreductase activity in bacterial cultures Chem. Commun. 2013; 49: 8815-8817.
[36] Chevalier A, Massif C, Renard P-Y, Romieu A. Bioconjugatable Azo-Based Dark-Quencher Dyes: Synthesis and Application to Protease-Activatable Far-Red Fluorescent Probes Chem.--Eur. J. 2013; 19: 1686-1699.
[37] Chevalier A, Hardouin J, Renard P-Y, Romieu A. Universal Dark Quencher Based on "Clicked" Spectrally Distinct Azo Dyes Org. Lett. 2013; 15: 6082-6085.
[38] Chevalier A, Dubois M, Le Joncour V, Dautrey S, Lecointre C, Romieu A, Renard P-Y, Castel H, Sabot C. Synthesis, Biological Evaluation, and in Vivo Imaging of the first Camptothecin-Fluorescein Conjugate Bioconjugate Chem. 2013; 24: 1119-1133.
[39] Priem T, Bouteiller C, Camporese D, Romieu A, Renard P-Y. Synthesis and reactivity of a bis-sultone cross-linker for peptide conjugation and [18F]-radiolabelling via unusual "double click" approach Org. Biomol. Chem. 2012; 10: 1068-1078.
[40] Niu S-L, Massif C, Ulrich G, Renard P-Y, Romieu A, Ziessel R. Water-Soluble Red-Emitting Distyryl-Borondipyrromethene (BODIPY) Dyes for Biolabeling Chem.--Eur. J. 2012; 18: 7229-7242.
[41] Massif C, Dautrey S, Haefele A, Ziessel R, Renard P-Y, Romieu A. New insights into the water-solubilization of fluorophores by post-synthetic "click" and Sonogashira reactions Org. Biomol. Chem. 2012; 10: 4330-4336.
[42] Traoré T, Clavé G, Delacour L, Kotera N, Renard P-Y, Romieu A, Berthault P, Boutin C, Tassali N, Rousseau B. The first metal-free water-soluble cryptophane-111 Chem. Commun. 2011; 47: 9702-9704.
[43] Romieu A, Bruckdorfer T, Clavé G, Grandclaude V, Massif C, Renard P-Y. N-Fmoc-a-sulfo-b-alanine: a versatile building block for the water solubilization of chromophores and fluorophores by solid-phase strategy Org. Biomol. Chem. 2011; 9: 5337-5342.
[44] Paunescu E, Louise L, Jean L, Romieu A, Renard P-Y. A versatile access to new halogenated 7-azidocoumarins for photoaffinity labeling: Synthesis and photophysical properties Dyes Pigm. 2011; 91: 427-434.
[45] Maindron N, Poupart S, Hamon M, Langlois J-B, Plé N, Jean L, Romieu A, Renard P-Y. Synthesis and luminescence properties of new red-shifted absorption lanthanide(III) chelates suitable for peptide and protein labelling Org. Biomol. Chem. 2011; 9: 2357-2370.
[46] Koci J, Grandclaude V, Massonneau M, Richard J-A, Romieu A, Renard P-Y. A novel and unusually long-lived chemiluminophore based on the 7-hydroxycoumarin scaffold Chem. Commun. 2011; 47: 6713-6715.
[47] Debunne M, Portal C, Delest B, Brakenhielm E, Lallemand F, Henry J-P, Ligeret H, Noack P, Massonneau M, Romieu A, Renard P-Y, Thuillez C, Richard V. In vitro and ex vivo evaluation of smart infra-red fluorescent caspase-3 probes for molecular imaging of cardiovascular apoptosis International Journal of Molecular Imaging 2011: Article ID 413290.
[48] Romieu A, Tavernier-Lohr D, Pellet-Rostaing S, Lemaire M, Renard P-Y. Water solubilization of xanthene dyes by post-synthetic sulfonation in organic media Tetrahedron Lett. 2010; 51: 3304-3308.
[49] Niu SL, Massif C, Ulrich G, Ziessel R, Renard P-Y, Romieu A. Water-solubilization and bio-conjugation of a red-emitting BODIPY marker Org. Biomol. Chem. 2010; 9: 66-69.
[50] Meyer Y, Richard J-A, Delest B, Noack P, Renard P-Y, Romieu A. A comparative study of the self-immolation of para-aminobenzylalcohol and hemithioaminal-based linkers in the context of protease-sensitive fluorogenic probes Org. Biomol. Chem. 2010; 8: 1777-1780.
[51] Louise-Leriche L, Paunescu E, Saint-Andre G, Baati R, Romieu A, Wagner A, Renard P-Y. A HTS Assay for the Detection of Organophosphorus Nerve Agent Scavengers Chem.--Eur. J. 2010; 16: 3510-3523.
[52] Clavé G, Volland H, Flaender M, Gasparutto D, Romieu A, Renard P-Y. A universal and ready-to-use heterotrifunctional cross-linking reagent for facile synthetic access to sophisticated bioconjugates Org. Biomol. Chem. 2010; 8: 4329-4345.
[53] Clavé G, Ronco C, Boutal H, Kreich N, Volland H, Franck X, Romieu A, Renard PY. Facile and rapid access to linear and truncated microcystin analogs for the implementation of immunoassays Org. Biomol. Chem. 2010; 8: 676-690.
[54] Ronco C, Sorin G, Nachon F, Foucault R, Jean L, Romieu A, Renard P-Y. Synthesis and structure-activity relationship of Huprine derivatives as human acetylcholinesterase inhibitors Bioorg. Med. Chem. 2009; 17: 4523-4536.
[55] Richard J-A, Jean L, Schenkels C, Massonneau M, Romieu A, Renard P-Y. Self-cleavable chemiluminescent probes suitable for protease sensing Org. Biomol. Chem. 2009; 7: 2941-2957.
[56] Niu SL, Ulrich G, Ziessel R, Kiss A, Renard P-Y, Romieu A. Water-soluble BODIPY derivatives Org. Lett. 2009; 11: 2049-2052.
[57] Turcatti G, Romieu A, Fedurco M, Tairi A-P. A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis Nucleic Acids Res. 2008; 36: e25/21-e25/13.
[58] Romieu A, Brossard D, Hamon M, Outaabout H, Portal C, Renard P-Y. Postsynthetic derivatization of fluorophores with alpha-sulfo-beta-alanine dipeptide linker. Application to the preparation of water-soluble cyanine and rhodamine dyes Bioconjugate Chem. 2008; 19: 279-289.
[59] Richard J-A, Meyer Y, Jolivel V, Massonneau M, Dumeunier R, Vaudry D, Vaudry H, Renard P-Y, Romieu A. Latent Fluorophores Based on a Self-Immolative Linker Strategy and Suitable for Protease Sensing†Bioconjugate Chem. 2008; 19: 1707-1718.
[60] Richard J-A, Massonneau M, Renard P-Y, Romieu A. 7-Hydroxycoumarin-Hemicyanine Hybrids: A New Class of Far-Red Emitting Fluorogenic Dyes Org. Lett. 2008; 10: 4175-4178.
[61] Meyer Y, Richard J-A, Massonneau M, Renard P-Y, Romieu A. Development of a New Nonpeptidic Self-Immolative Spacer. Application to the Design of Protease Sensing Fluorogenic Probes Org. Lett. 2008; 10: 1517-1520.
[62] Clavé G, Boutal H, Hoang A, Perraut F, Volland H, Renard P-Y, Romieu A. A novel heterotrifunctional peptide-based cross-linking reagent for facile access to bioconjugates. Applications to peptide fluorescent labelling and immobilisation Org. Biomol. Chem. 2008; 6: 3065-3078.
[63] Bentley DR et al. Accurate whole human genome sequencing using reversible terminator chemistry Nature 2008; 456: 53-59.
[64] Richard J-A, Jean L, Romieu A, Massonneau M, Noack-Fraissignes P, Renard P-Y. Chemiluminescent Probe for the in Vitro Detection of Protease Activity Org. Lett. 2007; 9: 4853-4855.
[65] Bouteiller C, Clavé G, Bernardin A, Chipon B, Massonneau M, Renard P-Y, Romieu A. Novel Water-Soluble Near-Infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes Bioconjugate Chem. 2007; 18: 1303-1317.
[66] Poupart S, Boudou C, Peixoto P, Massonneau M, Renard P-Y, Romieu A. Aminopropargyl derivative of terpyridine-bis(methyl-enamine) tetraacetic acid chelate of europium (Eu (TMT)-AP3): a new reagent for fluorescent labelling of proteins and peptides Org. Biomol. Chem. 2006; 4: 4165-4177.
[67] Lapeyre M, Leprince J, Massonneau M, Oulyadi H, Renard P-Y, Romieu A, Turcatti G, Vaudry H. Aryldithioethyloxycarbonyl (Ardec): a new family of amine protecting groups removable under mild reducing conditions and their applications to peptide synthesis Chem.--Eur. J. 2006; 12: 3655-3671.
[68] Fedurco M, Romieu A, Williams S, Lawrence I, Turcatti G. BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies Nucleic Acids Res. 2006; 34: e22/21-e22/13.
[69] Clavé G, Bernardin A, Massonneau M, Renard P-Y, Romieu A. Latent fluorophores based on a Mannich cyclisation trigger Tetrahedron Lett. 2006; 47: 6229-6233.
[70] Chipon B, Clavé G, Bouteiller C, Massonneau M, Renard P-Y, Romieu A. Synthesis and post-synthetic derivatization of a cyanine-based amino acid. Application to the preparation of a novel water-soluble NIR dye Tetrahedron Lett. 2006; 47: 8279-8284.
[71] Raguin O, Fournie-Zaluski M-C, Romieu A, Pelegrin A, Chatelet F, Pelaprat D, Barbet J, Roques BP, Gruaz-Guyon A. A labeled neutral endopeptidase inhibitor as a potential tool for tumor diagnosis and prognosis Angew. Chem., Int. Ed. 2005; 44: 4058-4061.
[72] Cadet J, Bellon S, Douki T, Frelon S, Gasparutto D, Muller E, Pouget J-P, Ravanat J-L, Romieu A, Sauvaigo S. Radiation-induced DNA damage: formation, measurement, and biochemical features J. Environ. Pathol. Toxicol. Oncol. 2004; 23: 33-43.
[73] Belliard G, Romieu A, Zagury J-F, Dali H, Chaloin O, Le Grand R, Loret E, Briand J-P, Roques B, Desgranges C, Muller S. Specificity and effect on apoptosis of Tat antibodies from vaccinated and SHIV-infected rhesus macaques and HIV-infected individuals Vaccine 2003; 21: 3186-3199.
[74] Dong C-Z, Romieu A, Mounier CM, Heymans F, Roques BP, Godfroid J-J. Total direct chemical synthesis and biological activities of human group IIA secretory phospholipase A2 Biochem. J. 2002; 365: 505-511.
[75] Luciani N, De Rocquigny H, Turcaud S, Romieu A, Roques BP. Highly sensitive and selective fluorescence assays for rapid screening of endothelin-converting enzyme inhibitors Biochem. J. 2001; 356: 813-819.
[76] Bellon S, Gasparutto D, Romieu A, Cadet J. 5-(phenylthiomethyl)-2'-deoxyuridine as an efficient photoreactive precursor to generate single and multiple lesions within DNA fragments Nucleosides, Nucleotides Nucleic Acids 2001; 20: 967-971.
[77] Romieu A, Bellon S, Gasparutto D, Cadet J. Synthesis and UV Photolysis of Oligodeoxynucleotides That Contain 5-(Phenylthiomethyl)-2'-deoxyuridine: A Specific Photolabile Precursor of 5-(2'-Deoxyuridilyl)methyl Radical Org. Lett. 2000; 2: 1085-1088.
[78] Muller E, Gasparutto D, Jaquinod M, Romieu A, Cadet J. Chemical and biochemical properties of oligonucleotides that contain (5'S,6S)-cyclo-5,6-dihydro-2'-deoxyuridine and (5'S,6S)-cyclo-5,6-dihydrothymidine, two main radiation-induced degradation products of pyrimidine 2'-deoxyribonucleosides Tetrahedron 2000; 56: 8689-8701.
[79] Kuraoka I, Bender C, Romieu A, Cadet J, Wood RD, Lindahl T. Removal of oxygen free-radical-induced 5',8-purine cyclodeoxynucleosides from DNA by the nucleotide excision-repair pathway in human cells Proc. Natl. Acad. Sci. USA 2000; 97: 3832-3837.
[80] Gasparutto D, Bourdat A-G, D'Ham C, Duarte V, Romieu A, Cadet J. Repair and replication of oxidized DNA bases using modified oligodeoxyribonucleotides Biochimie 2000; 82: 19-24.
[81] Cadet J, Bourdat AG, D'Ham C, Duarte V, Gasparutto D, Romieu A, Ravanat JL. Oxidative base damage to DNA: specificity of base excision repair enzymes Mutat. Res., Rev. Mutat. Res. 2000; 462: 121-128.
[82] Romieu A, Gasparutto D, Molko D, Ravanat J-L, Cadet J. Synthesis of oligonucleotides containing the (4R) and (4S) diastereoisomers of 4,8-dihydro-4-hydroxy-8-oxo-2'-deoxyguanosine Eur. J. Org. Chem. 1999: 49-56.
[83] Romieu A, Gasparutto D, Molko D, Cadet J. Synthesis and characterization of oligodeoxynucleotides containing 5',8-cyclopurine-2'-deoxyribonucleosides Nucleosides & Nucleotides 1999; 18: 1331-1333.
[84] Romieu A, Gasparutto D, Cadet J. Synthesis and characterization of oligodeoxyribonucleotides containing the two 5R and 5S diastereomers of (5'S,6S)-5',6-cyclo-5,6-dihydrothymidine; radiation-induced tandem lesions of thymidine J. Chem. Soc., Perkin Trans. 1 1999: 1257-1264.
[85] Romieu A, Gasparutto D, Cadet J. Synthesis and Characterization of Oligonucleotides Containing 5',8-Cyclopurine-2'-deoxyribonucleosides : (5'R)-5',8-Cyclo-2'-deoxyadenosine, (5'S)-5',8-Cyclo-2'-deoxyguanosine, and (5'R)-5',8-Cyclo-2'-deoxyguanosine Chem. Res. Toxicol. 1999; 12: 412-421.
[86] D'Ham C, Romieu A, Jaquinod M, Gasparutto D, Cadet J. Excision of 5,6-Dihydroxy-5,6-dihydrothymine, 5,6-Dihydrothymine, and 5-Hydroxycytosine from Defined Sequence Oligonucleotides by Escherichia coli Endonuclease III and Fpg Proteins: Kinetic and Mechanistic Aspects Biochemistry 1999; 38: 3335-3344.
[87] Cadet J, Douki T, Gasparutto D, Gromova M, Pouget JP, Ravanat JL, Romieu A, Sauvaigo S. Radiation-induced damage to DNA: mechanistic aspects and measurement of base lesions Nucl. Instrum. Methods Phys. Res., Sect. B 1999; 151: 1-7.
[88] Cadet J, Bardet M, Berger M, Berthod T, Delatour T, D'Ham C, Douki T, Gasparutto D, Grand A, Guy A, Jolibois F, Molko D, Polverelli M, Ravanat JL, Romieu A, Signorini N, Sauvaigo S. Oxidative base damage to DNA: Recent mechanistic aspects, measurement and repair NATO ASI Series, Series A: Life Sciences 1999; 302: 47-58.
[89] Romieu A, Gasparutto D, Molko D, Cadet J. Site-specific Introduction of (5'S)-5',8-Cyclo-2'-Deoxyadenosine into Oligodeoxyribonucleotides J. Org. Chem. 1998; 63: 5245-5249.
[90] Romieu A, Gasparutto D, Molko D, Cadet J. A convenient synthesis of 5-hydroxy-2'-deoxycytidine phosphoramidite and its incorporation into oligonucleotides Tetrahedron Lett. 1997; 38: 7531-7534.

Patents :

[1] Fedurco M, Romieu A, Turcatti G. Use of trimesic and benzenetriacetic acids to prepare carboxyl group-containing substrates for immobilization of biomolecules, Solexa Limited, Lynx Therapeutics Inc.
[2] Fedurco M, Romieu A, Turcatti G. Improved method of fluorescence-based nucleic acid sequencing in antioxidant-containing buffer, Solexa Limited.
[3] Renard PY, Romieu A, Massonneau M. Preparation of substituted 1,2-dioxetane biomarkers with luminescent emission and their use, Quidd, Université de Rouen.
[4] Liu X, Milton J, Smith GP, Barnes C, Rasolonjatovo IMJ, Rigatti R, Wu X, Ost TWB, Worsley GJ, Earnshaw DJ, Turcatti G, Romieu A. Preparation of single-stranded templates for nucleic acid sequencing, Solexa Limited.
[5] Renard PY, Romieu A, Richard JA, Massonneau M. Preparation of new pro-fluorescent compounds, particularly peptidyl coumarin and acridinone derivatives, as probes for the detection of protease activity, Quidd, Université de Rouen.
[6] Clavé G, Renard PY, Romieu A, Volland H. Preparation of trifunctional pseudopeptide reagents, especially luminescent reagents, and their bioconjugates, and their use for the functionalization of solid supports for the detection of biomolecules, Commissariat à l'Energie Atomique.
[7] Bouteiller C, Mariani MF, Ronco C, Renard PY, Jean L, Romieu A. Labeled huprine derivatives and their use in medical imaging, Université de Rouen, Advanced Accelerator Applications.
[8] Ronco C, Renard PY, Jean L, Nachon F, Romieu A. Preparation of 7,11-methanocycloocta[b]quinoline derivative as highly functionalizable acetylcholinesterase inhibitors for treating neurological diseases, Université de Rouen.
[9] Priem T, Bouteiller C, Camporese D, Romieu A, Renard P-Y. Preparation of new nucleophile-reactive sulfonated compounds for the radiolabeling of biomolecules; precursors and conjugates thereof, Advanced Accelerator Applications.
[10] Viault G, Dautrey S, Renard P-Y, Romieu A. Reagent including four orthogonal functions and its uses, Université de Rouen.
[11] Orenga S, Chalansonnet V, Chevalier A, Renard P-Y, Romieu A, Roubinet B. Fluorogenic/fluorescent probes derivative from sulfoxanthene, and use thereof, bioMérieux.

(1) Organic synthesis (multi-steps, photochemical, asymmetric) of various molecules: biomolecules, cleavable and/or traceless linkers, fluorophores (acridine, coumarin, cyanine, diazoic, DPP, oxazine and (hetero)xanthene), N-heterocycles (polypyridyl ligands) and organosilanes.

(2) Bio-organic chemistry: synthesis of modified nucleosides, nucleotides and amino acids. Post-synthetic modification (fluorescent labeling, introduction of PEG and/or cleavable linkers, or reactive functional groups: thiol, ...), photochemistry et enzymology of nucleic acids. Advanced organic chemistry in the context of peptide synthesis (cyclization, fluorescent labeling and vectorization of inhibitors). Bioconjugation techniques (wet chemistry) applied to nucleic acids and proteins (click chemistry, ...).

(3) Automated solid-phase synthesis: peptide synthesis ("Fmoc/tBu" strategy, ABI 431A, 433 and Symphony/Multiplex) and opligonucleotide synthesis ("phosphoramidite chemistry", ABI 392).

(4) Surface chemistry: cleaning (etching), silanization and functionalization of glass surfaces (formats: slides and microchannels).

(5) Analytical chemistry: purification by HPLC (RP, regular and ion-exchange), purification by size-exclusion chromatography (purification of proteins), HPLC, GC, GC-MS, LC-MS and ESI-MS (QUATTRO micro, Micromass, LCQ Advantage MAX (ion-trap) and MSQ Plus, Thermo Scientific) analyses, spectroscopic characterizations by IR and RMN 1D/2D (1H and heteronuclei), photophysics (Abs/Ex/Em spectra, absolute/relative fluorescence quantum yields, ETE). Development et validation of QC procedures for the analysis (purity and stability) of raw materials (chemical reagents).